Identification of cords by Mycobacterium kansassi and its implications in clinical microbiology
DOI:
https://doi.org/10.20453/rmh.v33i4.4406Keywords:
Tuberculosis, Mycobacterium kansasii, bacteria, disease, virulenceAbstract
Objectives: To determine if cord formation occurs in microcolonies of M. kansassi. Methods: Four species of highly prevalent pathogenic mycobacteria were seeded in the solid medium 7H11; Mycobacterium kansassi, Mycobacterium abscessus, Mycobacterium tuberculosis y Mycobacterium neonarum and observed for 21 days performing the Ziehl- Neelsen stain. Inverted light microscopy was used to detect cord formation. Results: All species produced cords, Mycobacterium kansassi, Mycobacterium abscessus, Mycobacterium tuberculosis y Mycobacterium neonarum at earlier stages of their growth detected by Ziehl-Neelsen. Conclusions: M kansasii is able to develop cords at microscopic levels indicating that this property is not distinctive of M tuberculosis as was originally thought.
References
Palomino J, Leao S, Ritacco V. Tuberculosis 2007 From basic science to patient care. Primera edición. Berlin: BourcillerKamps; 2007. (Citado el 18 de octubre del 2020) Disponible en: http://pdf.flyingpublisher.com/tuberculosis2007.pdf
Kremer K, Van Der Werf J, Au B, et al. Vaccine-induced Immunity Circumvented by typical Mycobacterium tuberculosis Beijing Strains. Emerg Infect Dis. 2009; 15(2): 335–339. doi: 10.3201/eid1502.080795
Shiferaw G, Woldeamanuel Y, Gebeyehu M, Demessie D, Lemma E. Evaluation of Microscopic Observation Drug Susceptibility Assay (MODS) Detection of Multidrug-Resistant Tuberculosis (MDR-TB). J Clin Microbiol. 2007; 45(4):1093-7. doi: 10.1128/JCM.01949-06
Moore D, Carlton A, Gilman R, et al. Microscopic-observation drug-susceptibility assay for the diagnosis of TB. N Engl J Med. 2006; 355(15):1539-50. doi: 10.1056/NEJMoa055524
Ernst J, Trevejo-Nuñez G, Banaiee N. Genomics and the evolution, patogénesis and diagnosis of Tuberculosis. J Clin Invest. 2007; 117(7):1738-45. doi: 10.1172/JCI31810
Selvarangan R, Wu W, Nguyen T, et.al. Characterization of a Novel Group of Mycobacteria and Proposal of Mycobacterium sherrisii sp. nov. J Clin Microbiol. 2004; 42(1):52-9. doi: 10.1128/jcm.42.1.52-59.2004
Fregnan G, Smith D, Randall H. Biological and Chemical Studies on mycobacteria relationship of colony morphology to mycoside content for Mycobacterium kansasii and Mycobacterium fortuitum. J Bacteriol. 1961; 82(4):517-27. doi: 10.1128/JB.82.4.517-527.1961
Byrd T, Lyons R. Preliminary Characterization of a Mycobacterium abscessus Mutant in Human and Murine Models of Infection. Infect Immun. 1999; 67(9):4700-7. doi: 10.1128/IAI.67.9.4700-4707.1999
REMEL. Middlebrook 7h11 agar (Thin pour). Lenexa, Kansas, USA: REMEL; 2011. (Citado el 18 de octubre del 2020) Disponible en: https://assets.thermofisher.com/TFS-Assets/LSG/manuals/IFU1606-PI.pdf
Dorronsoro I, Torroba L. Microbiología de la tuberculosis. An Sist Sanit Navar. 2007; 30(2):67-84.
Sánchez-Chardi A, Olivares F, Byrd T, Julián E, Brambilla E, Luquin M. Demonstration of cord formation by rough Mycobacterium abscessus variants: implications for the clinical microbiology laboratory. J Clin Microbiol. 2011; 49(6):2293-5. doi: 10.1128/JCM.02322-10
Hee-Jae Huh, Su-Young Kim, Byung-Woo Jhun, Sung-Jae Shin, Won-Jung Koh. Recent advances in molecular diagnostics and understanding mechanisms of drug resistance in nontuberculous mycobacterial diseases. Meegid. 2018; 72(1):1-71. doi:10.1016/ j.meegid.2018.10.003
Attorri S, Dumbar S, Clarridge J. Assessment of Morphology for Rapid Presumptive Identification of Mycobacterium tuberculosis and Mycobacterium kansasii. J Clin Microbiol. 2000; 38 (4):1426–29.
Tu H, Chang S, Huaug T, Huaug W, Liu Y, lee S. Microscopic Morphology in Smears Prepared from MGIT Broth Medium for Rapid Presumptive Identification of Mycobacterium tuberculosis complex, Mycobacterium avium complex and Mycobacterium kansasii. Ann Clin Lab Sci. pring 2003; 33(2):179-83.
Moon SM, Park HY, Jeon K, im S-Y, Chung MJ, Huh HJ, et al. Clinical Significance of Mycobacterium kansasii Isolates from Respiratory Specimens. PLoS ONE. 2015; 10(10): e0139621. doi: 10.1371/journal.pone.0139621
Rojas Jaimes J, Giraldo-Chavez J, Huyhua-Flores Y, Caceres-Nakiche T. Identificación de micobacterias en medio solido mediante microscopía de fase invertida y tinción Ziehl-Neelsen. Rev Peru Med Exp Salud Publica. 2018; 35(2):279-84. doi:10.17843/rpmesp.2018.352.3471
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