Porphyromonas gingivalis in crevicular fluid of type 2 diabetic patients.

Authors

  • Maria Rosenda Britos Faculty of Dentistry. National University of the Northeast. Corrientes, Argentina.
  • Cynthya Solange Sin Microbial Biotechnology for Food Innovation (BiMIA) - (IMIT) Institute of Modeling and Technological Innovation - CONICET. Faculty of Dentistry. National University of the Northeast. Corrientes, Argentina.
  • Silvia Mercedes Ortega Microbial Biotechnology for Food Innovation (BiMIA) - (IMIT) Institute of Modeling and Technological Innovation - CONICET. Faculty of Dentistry. National University of the Northeast. Corrientes, Argentina.

DOI:

https://doi.org/10.20453/reh.v32i1.4181

Keywords:

Porphyromonas gingivalis, Mellitus Diabetes type 2, blood glucose

Abstract

Objective: To obtain the prevalence of Porphyromonas gingivalis in patients with type 2 Diabetes Mellitus. Material and method: This study was descriptive and cross-sectional. The type of sampling was for convenience and the sample consisted of 50 patients diagnosed with Type 2 Diabetes Mellitus. The degrees of Periodontitis were classified according to the criteria of Papapanou et al., (2018). The prevalence of Porphyromonas gingivalis was obtained by end-point PCR. The sample was taken in two places with greater depth of pocket. Glucose control was evaluated by measuring the percentage of glycosylated hemoglobin. The statistical analysis was performed using the InfoStat 2019 Software and the Independence Test was used using the Chi-Square statistic with 5% significance. Results: A 30% prevalence of P. gingivalis was obtained. 56% of the sample had a grade 0 of periodontitis, 24% grade I, 8% had grade II and 12% had grade III. No statistically significant difference was found between the presence of P. gingivalis and the degrees of periodontitis. Conclusions: The prevalence of P. gingivalis in the sample of patients with type 2 diabetes is 30% and its distribution is independent of the degree of periodontal disease.

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References

Devine DA, Marsh PD, Meade J. Modulation of host responses by oral comensal bacteria. J Oral Microbiol. 2015; 7:26941. DOI:10.3402/jom.v7.26941

Takahashi N. Oral Microbiome Metabolism: From “Who Are They?” to “What Are They Doing?”. J Dent Res. 2015;94(12):1628-37.

Socransky SS, Haffajee AD. The bacterial etiology of destructive periodontal disease: current concepts. Journal of periodontology. 1992; 63: 322-331.

Socransky SS, Haffajee AD. Periodontal microbial ecology. Periodontology. 2000; 38: 135-187.

Armitage GC. Development of a classification system for periodontal diseases and conditions. Ann Periodontol. 1999;4(1):1-6. doi: 10.1902/ annals.1999.4.1.1

Gomis G, Serva O. Diabetes y enfermedad periodontal. FMC .2017;24(2):64-9.

Britos MR, Sin CS, Ortega SM. Enfermedad periodontal y su implicancia en la diabetes mellitus: revisión de la literatura. Rev Ateneo Argent Odontol. 2019;60(1): 33- 40.

López M, Diaz M. La diabetes mellitus y su vinculación en la etiología y patogenia de la enfermedad periodontal. Gaceta medica espirituana. 2017; 9(2). (Citado el 10 de junio del 2021). Disponible en: http:// revgmespirituana.sld.cu/index.php/gme/article/ view/794/640

Jaramillo A. Asociación entre síndrome metabólico y enfermedad períodontal en personas que asisten a 5 instituciones de salud en Cali, Medellín y Bogotá.Tesis de Grado. Colombia: Universidad del Valle; 2017. Citado el 10 de junio del 2021). Disponible en: http:// hdl.handle.net/10893/10110

Arana C, Florencio L, Sevillano M, et al. Diabetes and periodontal diseases: an established two-way relationship. Journal of Diabetes Mellitus, 2016; 6(4):209-229 DOI:10.4236/JDM.2016.64024

DeMarziani G, Elbert AE. Hemoglobina glicada (HbAa1c). Utilidad y limitaciones en pacientes con enfermedad renal crónica. Revista de Nefrología, Diálisis y Trasplante. 2018; 38(1): 65-83.

Asociación Latinoamericana de Diabetes. Guías ALAD sobre el diagnóstico, control y tratamiento de la diabetes mellitus tipo 2 con medicina basada en evidencia. Revista de la ALAD; 2019. (Citado el 10 de junio del 2021). Disponible en: https://www. revistaalad.com/guias/5600AX191_guias_alad_2019. pdf

Mulhall H, Huck O, Amar S. Porphyromonas gingivalis, a Long-Range Pathogen: Systemic Impact and Therapeutic Implications. Microorganisms. 2020;8(6):869. doi: 10.3390/microorganisms8060869

Moreno MC, Valladares-García J, Halabe-Cherem J. Microbioma humano. Revista de la Facultad de Medicina UNAM. 2018;61(6):7-19. (Citado el 10 de junio del 2021). Disponible en: https://www. medigraphic.com/pdfs/facmed/un-2018/un186b.pdf

Rocha VH, Nobre dos Santos L E, Montino AC, et al. Porphyromona gingivalis and Chronic Periodontitis. Recent advances. Revista Bahiana de Odontología, 2016;7(2):147-154. (Citado el 10 de junio del 2021). Disponible en: https://www5.bahiana.edu.br/index. php/odontologia/article/view/885/633

Seyama M, Yoshida K, Yoshida K, et al. Outer membrane vesicles of Porphyromonas gingivalis attenuate insulin sensitivity by delivering gingipains to the liver. Biochim Biophys Acta Mol Basis Dis. 2020;1866(6):165731. doi: 10.1016/j.bbadis.2020.165731

Tian J, Liu C, Zheng X, et al. Porphyromonas gingivalis Induces Insulin Resistance by Increasing BCAA Levels in Mice. J Dent Res. 2020;99(7):839- 846. doi: 10.1177/0022034520911037

Yang R, Dong J, Zhao H, et al. Association of branched- chain amino acids with carotid intima-media thickness and coronary artery disease risk factors. PLoS ONE. 2014;9:e99598.

Takamura H, Yoshida K, Okamura H, Fujiwara N, Ozaki K. Porphyromonas gingivalis attenuates the insulin-induced phosphorylation and translocation of forkhead box protein O1 in human hepatocytes. Archives of Oral Biology. 2016; 69: 19-24.

Papapanou PN, Sanz M, Buduneli N, et al. Periodontitis: Consensus report of workgroup 2 of the 2017 World Workshop on the Classification of Periodontal and Peri- Implant Diseases and Conditions. J Periodontol. 2018;89 Suppl 1:S173-S182. doi: 10.1002/JPER.17- 0721

Quintero AJ, Prada P, Inostroza CM, et al. Presencia de Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola y Aggregatibacter actinomycetemcomitans en el biofilm subgingival de pacientes diabéticos tipo 2: estudio transversal. Rev Clin Periodoncia Implantol Rehabil Oral 2011; 4:54- 58. (Citado el 10 de junio del 2021). Disponible en: www.scielo.cl/pdf/piro/v4n2/art03.pdf

Correa S, Torres J, Echeverry AJ, Rengifo AC. Frecuencia de los genotipos de FimA de Porphyromonas gingivalis, Tanerella forsythia, Treponema denticola y Aggregatibacter actinomycetemcomitans en pacientes diabéticos con enfermedad periodontal/Frequency of FimA Genotypes of P. gingivalis. Universitas Odontológica. 2015; 34(73):129-138.

Sánchez MB. Determinación de las características clínicas, radiográficas y microbiológicas de la enfermedad periodontal en pacientes diabéticos tipo I y tipo II de la asociación de diabéticos juveniles y del patronato para diabéticos de Guatemala (Papadigua), en el año 2008.Tesis doctoral. Guatemala: Universidad de San Carlos de Guatemala; 2010.

Jaques GA, Maldonado JI, Lobos O. Prevalencia y concentración de Porphyromonas gingivalis en fluido crevicular y saliva de pacientes diabéticos. Tesis doctoral. Talca, Chile: Universidad de Talca; 2019.

Tervonen T, Oliver RC, Wolff LF, Bereuter J, Anderson L, Aeppli DM. Prevalence of periodontal pathogens with varying metabolic control of diabetes mellitus. J Clin Periodontol. 1994;21(6):375-9.

Cordovez A G. Prevalencia de periodontitis y su grado de severidad en pacientes del grupo de diabéticos del Hospital Vozandes Quito. Tesis de Bachiller. Quito: Universidad de las Américas; 2018.

Published

2022-04-22

How to Cite

1.
Britos MR, Sin CS, Ortega SM. Porphyromonas gingivalis in crevicular fluid of type 2 diabetic patients. Rev Estomatol Herediana [Internet]. 2022 Apr. 22 [cited 2024 Jul. 22];32(1):36-41. Available from: https://revistas.upch.edu.pe/index.php/REH/article/view/4181